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. 2022 Dec 23;324(2):H212–H225. doi: 10.1152/ajpheart.00490.2022

Figure 6.

Figure 6.

Diabetes-like conditions increased IREB2 expression to a greater extent in male cells (A) compared with female cells (B), as well as to markers of apoptosis (caspase 3) and necroptosis (RIPK3), especially under hypoxia environment. C: treatment of cells with ferroptosis inducer erastin did not affect FHC in either female or male cells, but there was an increase in FHC in male cells treated with erastin plus ferroptosis inhibitor Fer-1 ($P < 0.05). D: GPX4 levels were similar in control cells. Erastin lowered GPX4 in male cells ($P = 0.04) but did not affect female cells. Fer-1 recovered GPDX4 levels in male cells (#P = 0.0041 vs. erastin). E: IREB2 levels were greater in male cells in control and erastin groups ($P = 0.001). Fer-1 lowered IREB2 in male cells to levels observed in female cells. Results are shown as means ± SE of 3 individual experiments in triplicate. For C–E, three-way ANOVA was used. FHC, ferritin heavy chain; GPX4, glutathione peroxidase 4; IREB2: iron responsive element-binding protein 2; RIPK3, receptor interacting serin/threonine kinase 3.