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. 2022 Dec 23;324(2):H212–H225. doi: 10.1152/ajpheart.00490.2022

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Published by the American Physiological Society.

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Figure 7. — Hemin stimulates ferroptosis in female human immortalized BMVEC line (hCMEC/D3). A: representative images showing increased lipid peroxidation by hemin, which was prevented by Fer-1. B: hemin lowered cell survival. DFX improved survival in both control and hemin-treated cells. Hemin decreased GPX4 (C) and NRF2 (D) levels but increased FHC (E) levels. DFX treatment had differential effects by decreasing GPX4 and NRF2 levels in control cells but increasing those in hemin-treated cells. DFX did not affect the FHC level. ACSL4 level (F) had no significant change in all groups. Representative images (G) were obtained from three individual experiments in triplets. Results are shown as means ± SE of 3 individual experiments in triplicate. All panels were analyzed with two-way ANOVA. ACSL4, acyl-CoA synthetase long-chain family member 4; DFX, deferoxamine; GPX4, glutathione peroxidase 4; FHC, ferritin heavy chain; IHC, immunohistochemistry; NRF2, nuclear factor erythroid 2-related factor 2.