Skip to main content
. Author manuscript; available in PMC: 2023 Jan 23.
Published in final edited form as: Bioconjug Chem. 2022 Mar 18;33(4):544–554. doi: 10.1021/acs.bioconjchem.2c00057

Figure 3.

Figure 3.

(a) Colonies of Candida albicans showing deep-red spots due to cleavage of chromogenic substrate 2 within the agar by the HEX(NAG) enzyme produced by the fungus. (Reprinted (adapted) with permission,59 copyright (2002) (b) Cultured cells showing purple color due to cleavage of added chromogenic substrate 3 by HEX(NAG); (left) Sandhoff fibroblasts lacking HEX(NAG) activity (scale bar = 160 mm) and (right) transfected Sandhoff fibroblasts with elevated HEX(NAG) activity (scale bar = 40 mm), reprinted with permission from Springer Nature.64