FIG. 3.

TNF-α mRNA in ankles. Ankle mRNA transcripts were amplified by RT-PCR from rats fed either control (joint-control) or glycine (joint-glycine) diet 1 day after reactivation of arthritis using PG-PS. Ankles were harvested, and RNA was isolated as described in Materials and Methods. Markers (1.0 kb and 100 bp) and positive controls specifically for TNF mRNA and G3PDH, the housekeeping gene, were assayed simultaneously. The data are representative of two independent experiments.