FIG. 5.

Glycine receptor mRNA in splenic macrophages. Total RNA was isolated from splenic macrophages as described in Materials and Methods. The glycine receptor was identified by RT-PCR using primers specific for conserved regions in the beta subunit, (GlyRβ). The negative control was total RNA from splenic macrophages without RT. The PCR product was confirmed by sequencing as described in Materials and Methods. The data are representative of three independent experiments. Sizes are indicated in base pairs.