FIG. 6.

Effects of PG-PS and glycine on nuclear translocation of the p65 subunit of NF-κB. Splenic macrophages were stimulated with PG-PS (20 μg/ml) in the presence or absence of glycine (1 mM). The p65 subunit in cells was detected with an anti-p65 antibody as described in Materials and Methods. Nuclear DNA was stained using Hoechst 33342, and the p65 subunit and nuclear DNA were visualized with a fluorescence microscope. The data are representative of three independent experiments. Top row, untreated cells; middle row, cells treated with PG-PS (20 μg/ml) plus 5% rat serum; bottom row, glycine-pretreated cells treated with PG-PS.