Effect of glycine on superoxide production. Splenic macrophages were incubated in MEM with 15% FBS, HEPES, and antibiotics, and superoxide production was measured as described in Materials and Methods. Briefly, glycine (1 mM) was added to cells 3 min before the addition of PG-PS (10 μg/ml). Data are the means ± SEM from four independent experiments. ∗, P < 0.05 by one-way ANOVA with Tukey's post-hoc test compared with control group; #, P < 0.05 by one-way ANOVA with Tukey's post-hoc test compared with PG-PS alone.