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. Author manuscript; available in PMC: 2023 Jan 23.
Published in final edited form as: Methods Enzymol. 2022 Mar 18;666:297–314. doi: 10.1016/bs.mie.2022.02.003

Fig. 3.

Fig. 3

X-band CW EPR spectra of Cu2+ complexes at the His96 (GGGTH) and His111 (KTNMKHMAGA) sites, at pH6.5 and 8.5. Spectral changes upon pH are associated to the conversion of a 3N1O equatorial coordination mode to a 4N, as drawn. The pKa associated to this conversion is 7.8 for the His96 site and 7.5 for the His111 site.