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EPR methods and their applicability in Bottom up vs Top down investigations.
| EPR method | Bottom up (peptides) | Top down (proteins) |
|---|---|---|
| Continuous Wave (CW) | ✓✓ | ✓ |
| ESEEM | ✓✓ | ✓✓ |
| Isotope edited ESEEM and HYSCORE | ✓✓ | * |
| DEER Nitroxide-Nitroxide | ✓ | ✓✓ |
| DEER Copper-Nitroxide | ✓ | ✓✓ |
| Pulsed ENDOR | ✓ | ✓✓ |
✓✓: ideally suited; ✓: suitable but with limitations; *: requires specialized sample design.
