Extended Data Fig. 3. GC33-bbz-CAR-transduced-iPS-T cells did not show a remarkable difference from GC33-28bbz-CAR-transduced-iPS-T cells in tumor suppressive function.
a. Schematic presentation of CAR constructs GC33-28bbz and GC33-bbz CAR b. Cytokine production of GC33-28bbz and GC33-bbz CAR-transduced-iPS-T cells 48 h after co-culturing with irradiated SK-Hep-GPC3 (n = 4, mean ± SEM). Two-sided Student’s t test. c. NSG mice were injected subcutaneously with 2 × 105 JHH7 cells 3 days before the treatment. In total, 1 × 107 GC33-28bbz-iTCTL or GC33-bbz-iTCTL were injected intravenously on days 0 and 7 (n = 4 for each group). Tumor volume of the inoculated JHH7 at the indicated time points in individual mice treated with indicated test cells. n.s., not significant; SEM, standard error of the mean.