Extended Data Fig. 4. Butyrate micelle treatment reduced the anaphylactic response to peanut challenge in a dose-dependent manner.
a, b, Experimental schema, and the dosing strategy. All the mice were sensitized weekly by i.g. gavage of 6 mg of PN plus 10 μg of the mucosal adjuvant cholera toxin (CT). c, A uniform allergic response was confirmed by challenging one group of mice (n = 7) with 1 mg of PN i.p. and measuring reduction in core body temperature as an indication of anaphylaxis. QD: once a day. d-h, The rest of mice were randomized into five treatment groups and received either PBS (n = 12), ButM (n = 12), ButM (half dose) (n = 11), Neg-ButM only (n = 11), or NtL-ButM only (n = 12). d, Change in core body temperature following challenge with peanut extract. The area under curve (AUC) was compared among groups. e-h, Serum mMCPT-1 (e), histamine (f), peanut-specific IgE (g), and peanut-specific IgG1 (h) from mice in d. Data represent mean ± s.e.m. Data analyzed using one-way ANOVA with Dunnett’s post-test.