Extended Data Fig. 6. Butyrate micelle treatment did not significantly affect regulatory T cell (Treg) populations in spleen or mesenteric LNs.
a, Experimental schema. All of the vancomycin-treated mice were sensitized weekly by i.g. gavage of 6 mg of PN plus 10 μg of the mucosal adjuvant cholera toxin. The mice were then intragastrically treated with either PBS (n = 7), sodium butyrate (NaBut, n = 6), or ButM (n = 7) twice daily for two weeks. The mice were euthanized after treatment, and their spleen (b, c), ileum draining LNs (d, e), and colon draining LNs (f, g) were harvested and processed for flow cytometric analysis. Gating strategy is shown in Supplementary Fig. 19. Data represent mean ± s.e.m. Data analyzed using one-way ANOVA with Dunnett’s post-test.