FIG. 1.

The Igl, Hgl, and Lgl lectin subunits associate on the surface of amebic trophozoites. (a to c) Amebic trophozoites adhered to laminin-coated coverslips were fixed with paraformaldehyde and stained for Igl with MAb EH3015 and a secondary FITC-conjugated anti-mouse IgG antibody (green) (a) or polyclonal rabbit anti-Hgl-Lgl subunit antibodies and a secondary Cy3-conjugated anti-rabbit IgG antibody (red) (b). The merged image (yellow) is shown in panel c. (d to f) The 260-kDa subunit was first capped on the surface of trophozoites in suspension culture at 37°C with rabbit anti-260-kDa subunit antibodies. The amebae were then fixed and reacted with the anti-Igl MAb EH3015 and stained as in panels a to c.