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. 2023 Jan 23;3:3. doi: 10.1038/s43705-022-00202-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Venn diagram showing the percentage of significantly differentially expressed ORFs between mat types. B Differential expression of ORFs between orange and black mat pseudo replicates (12/18/16, 1/9/17 and 1/28/17). The following facet labels represent the collection of pathway-critical genes, referenced in Chan et al. [85] to depict functional processes: Nutrient limitation: “Phosphate limitation response”, “Nitrogen limitation response”. The following genes were used for detection of phosphate limitation: Alkaline phosphatase response regulators, high affinity phosphate-specific ABC transport genes, phoA, phoB, pstSCAB. For nitrogen limitation the following genes were used: glutamine synthase and regulators glnA, glnR, tnrA. Nitrogen cycling: Nitrogen fixation “Nif”, “Urease”. Carbon cycling “Photoautotrophy”, “Carbohydrate degradation”. Stress responses: Superoxide Dismutase “SOD”, “Peroxiredoxin”, “Heat shock protein” and “Osmoprotectants”. Significantly differentially expressed ORFs are colored by taxonomy and scaled by abundance. C Library-normalized expression of (top) nitrogen fixation, (middle) urease, and (bottom) antifreeze ORFs from black and orange mats over time. This analysis highlights the expression of several individual genes over at the single time point level. Additionally no orange mats were present in the streams at the November time point.