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. 2023 Jan 23;14:366. doi: 10.1038/s41467-023-36014-0

Fig. 5. Divergent expression of TAFA4 across species.

Fig. 5

a Dot plots showing the fraction of nuclei (size) and their scaled average expression level (color) in each neuron subtype (columns) across mouse, guinea pig, cynomolgus monkey, and human for genes (row names). NP denotes non-peptidergic; PEP denotes peptidergic. b Representative images of RNAScope validation of FAM19A4/TAFA4, NTRK2, and TUBB3 (mouse, guinea pig, human) or SNAP25 (cynomolgus monkey) in DRGs. Neurons are outlined in turquoise. White arrowheads indicate neurons (TUBB3+ or SNAP25+) expressing both TAFA4 and NTRK2 in human samples. Asterisks in the human images represent lipofuscin aggregates. Scale bar represents 50 µm. GP denotes guinea pig; Cyno denotes cynomolgus monkey. c Percentage of FAM19A4/TAFA4+, NTRK2+, and TAFA4+/NTRK2+ (‘double +’) in mouse, guinea pig, cynomolgus monkey, and human. d Size distribution of FAM19A4/TAFA4+, NTRK2+, and TUBB3+ or SNAP25+ cells in each species. Colored solid lines represent smoothed distributions of binned data. Gray dashed lines represent the 33% and 67% percentiles of the TUBB3+ or SNAP25+ distribution as a surrogate for small, medium, and large diameter neurons. Source data are provided as a Source Data file.