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. 2022 Dec 16;12:21768. doi: 10.1038/s41598-022-26335-3

Figure 2.

Figure 2

Slug EXs content analysis. (a) Comparison of the EXs protein concentrations provided by BCA and CBQCA quantification assays. (b) RNA content of the slug EXs. (c) RNA isolated from the slug EXs (lane 3) compared to loading control siRNA (21mer duplex with dTdT overhang, Sigma-Aldrich; lane 2) and to the DNA ladder (lowest band = 250 bp; lane 1). (d) Western blot analysis of slug EXs lysate using a Galectin-1/LGALS1 primary antibody against human galectin-1 protein (for the uncropped western blots and gels see supplemental data).