Figure 3.

Slug EXs loading with DOX. (a) After co-incubation of DOX with EXs for 2 h at 37 °C, (1) two subsequent washing steps were applied to remove the residual unbound DOX. The mixture was ultracentrifuged for 30 min at 100,000×g, the supernatant containing the free DOX was discarded, the pellet containing the nanovesicles washed with PBS, resuspended in 1 ml of PBS, and this step was repeated (2.–3.) before quantification of the EXs-bound DOX by LC–MS/MS (4). (b) Resulting uptake of the DOX per 10¹⁰.