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. 2023 Jan 23;9:7. doi: 10.1038/s41526-023-00248-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a After follicle culturing with Apocynin under SMG condition, the follicle-released oocytes showed a dramatically decreased ROS level compared to the oocytes without Apocynin. Scale bars, 100 μm. b The statistical analysis of DCF fluorescence intensity, showing a decreased ROS level in oocytes of the Apocynin group (n = 22 in NG, n = 26 in SMG and n = 41 in SMG + Apocynin). NG v.s. SMG: p value = 0.00000000000010, SMG v.s. SMG + Apocynin: p value = 0.000000028. c The ratio of PB1 (arrowheads) was markedly increased in the Apocynin-treated group compared to that in the SMG group. Scale bars, 100 μm. d Statistic analysis showing a significantly increased ratio of PB1 in the SMG + Apocynin group compared to that in the SMG group (n = 38 in NG, n = 35 in SMG and n = 43 in SMG + Apocynin). NG vs SMG: p value = 0.00021, SMG vs SMG + Apocynin: p value = 0.0019. Representative images of oocytes are shown. Data are presented as the mean ± SD. Data were analyzed by two-way ANOVA and n.s. P ≥ 0.05, **P < 0.01, ***P < 0.001.