Figure 1. PmPks1 and PmFmo3-1 are coexpressed during sea star development.

Confocal images show the expression pattern of PmPks1 and PmFmo3-1 using double FISH. Both genes are expressed in the same mesodermal cells located in the archenteron during gastrulation. Between the mid gastrula and late gastrula stages, PmPks1 and PmFmo3-1 expression shifts from mesodermal cells to cells scattered throughout the ectoderm. Some cells in the foregut also express both genes by the late gastrula stage. During the larval stages, ectodermal expression remains consistent while foregut expression is lost by the late larval stage. Nuclei are shown in blue with DAPI. Scale bars are 50 μm.