Figure 4. PmPks1 expression colocalizes with PmSix3 expression at early but not later stages of development.

Confocal images show the expression pattern of PmPks1 and PmSix3 using double FISH. At the early gastrula stage, PmSix3 is expressed in the invaginating vegetal pole and the anterior domain of the embryo. Anterior expression of PmSix3 is present until the late larval stage. PmPks1 is also expressed in the vegetal pole during the early gastrula stage, however not the anterior region. By the mid gastrula stage, PmPks1 and PmSix3 transcripts are detected in the same cells in the archenteron. Following this stage, colocalization of PmSix3 and PmPks1 is lost, as PmSix3 expression becomes restricted to the coelomic pouches and PmPks1 expression shifts to the ectoderm. Nuclei are shown in blue with DAPI. Scale bars are 50 μm.