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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Dev Biol. 2022 Nov 26;494:13–25. doi: 10.1016/j.ydbio.2022.11.008

Figure 5. Notch signaling is required to establish mesodermal PmPks1 expression.

Figure 5.

Confocal images show the expression pattern of PmPks1 and PmErg using double FISH at the mid gastrula stage. Delta/Notch signaling was inhibited by treating embryos with 32 μM DAPT at the 2 cell stage. Control embryos received an equivalent volume of DMSO. In control embryos, cells express either PmPks1 or PmErg in the archenteron. When Notch is inhibited, PmPks1 expressing cells are lost and the vast majority of mesodermal cells express PmErg. Nuclei are shown in blue with DAPI. Scale bars are 50 μm.