Fig. 4.

Longitudinal changes in hippocampal microglia: (A) Immunofluorescence staining for Iba1 staining in the PolyI:C treated mice. (B) Dynamic changes in microglia soma size, perimeter and circularity were quantified in Saline(NN) and PolyI:C (PP) mice in different age groups. (n = 3–4 mice per group & condition, data points come from 4 equidistant planes and at least 5 ROI`s/animal,*p < 0.05, two-way anova followed by Fisher's LSD test) (C) Bar chart representing the effect of treatment and aging on the average endpoints maximum branch length per cell (n = 3 mice per age group and condition, *p < 0.05, two-way ANOVA, followed by Benjamini Krieger and Yekutieli post hoc analyses, #p = 0.09) (D) Example of fractal analysis of microglia from the hippocampus of PolyI:C and saline treated 16 months aged mice. 63x single cell fractal analysis indicate the surface area (E) and perimeter (F) were decreased in PP mice, in contrast to the span ratio (G) showed a higher trend. PP mice showed a significant decrease in cell circularity (H) and fractal dimension (I), n = 45 cells from 3 mice per group and condition, mean ± SEM. #p = 0.07,*p < 0.05, **p < 0.005 Student's t-test. Scale bar, A = 20 μm.