Fig. 6.

Effect of PolyI:C on the hippocampal Lcn-2 & lipid droplet (LD) density. (A) Representative Lcn-2 stained confocal images of saline and PolyI:C treated mice in 9 months and 16 months aged PP mice. Larger aggregates of Lcn-2 (insert) visible at the 16 months PolyI:C exposed mice. (B) Quantification of % stained area indicates increased Lcn2 expression in the hippocampal CA3 field, *p < 0.05, two-way ANOVA followed with Holm-Sidak`s multiple comparison (n = 4–6 mice for 9 and 16-months groups/treatment). (C) Nile red positive lipid droplet staining (violet) across aging in NN & PP mice. Inserts showing aggregated lipid droplets (D) Representative triple fluorescence labeling with Nile Red, Iba1 (teal) and NF200 (grey) shows overlap between the different marker localized lipid droplets in Iba1 positive cells. Arrows indicating NileRed⁺/Iba1⁺ cells. (E) Bar chart representing the lipid droplet (LD) density, mean size and % stained area per ROI measured. Data are represented as mean ± SEM (n = 3–4 mice per groups/treatment). *p < 0.05, **p < 0.005, two-way ANOVA, Fischer LSD. Scale bar A = 20 μm, C,E = 10 μm.