Table 3. List of primers used for detecting specific RNAs using real-time PCR .

Gene	Sequence	Annealing	Fragment length
GAPDH	F: 5′-TTGACCTCAACTACAGGTTTACA -3′ R: 5′-GCTCCTCCTGGAAGATGGTGATG -3′	60°C	100
GPX4	F: 5′-TGGGAAATGCCATCAAGTGGA-3′ R: 5′-GGGCAGGTCCTTCTCTATCAC-3′	60°C	123
AKT1	F: 5′-GAGTTCACGGCCCAGATGAT-3′ R: 5′-CGAGTAGGAGAACTGGGGGA-3′	57°C	105
Nrf2	F: 5′-ATGCCACAGGACATTGAGCA-3′ R: 5′-TTGGCTTCTGGACTTGGAAC-3′	60°C	119
CoQ10B	F: 5′-TTGGATTTCCACCTGTGTTG-3′ R: 5′-CGCCAAATAGTCTCCAAATGA-3′	59°C	118

F: forward primer, R: reverse primer.