Table 3.

Summary of Data in BRB12 buffer

Construct	RL (μm)	Vel (μm/s)	${\mathrm{k}}_{\mathrm{o}\mathrm{n}}^{\mathrm{M}\mathrm{t}}$ (μM−1s−1)
1A(1-393)	14.3 ± 0.4	1.1 ± 0.02a	32 ± 4.4
1A-K1L12	6.7 ± 0.1	1.6 ± 0.02a	6.9 ± 2.4
4Q	2.6 ± 0.1	1.0 ± 0.05a	6.6 ± 5.5
5Q	1.2 ± 0.1	0.6 ± 0.03a	7.0 ± 1.2
P305L	2.9 ± 0.1	0.7 ± 0.02a	9.3 ± 3

^aSegmented Velocity reported.