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. Author manuscript; available in PMC: 2023 Jan 24.
Published in final edited form as: Methods Enzymol. 2022 Nov 24;679:191–233. doi: 10.1016/bs.mie.2022.08.050

Figure 9.

Figure 9.

MALDI-TOF MS data for in vitro activity assay using the thiomuracin RRE domain (TbtF). (A) Reaction scheme for thiazole/thiazoline formation on the thiomuracin precursor peptide. Cysteine residues highlighted in purple are cyclized by TbtG in an RRE-dependent process, and further oxidized by TbtE. (B) MALDI-TOF mass spectra showing the results of leaving individual reaction components out during in vitro activity assays. As shown, the TbtG cyclization reaction is dependent on the RRE (TbtF) as well as ATP. Figure adapted from (Hudson et al., 2015).