Table 3.

Number of validated cloned sequences, single nucleotide variants and alleles of NR1 and NR2 genes in Giardia assemblage B isolates.

	Nitroreductase 1					Nitroreductase 2
	Sequenced clonesa	SNVs	nsSNVs	nsSNVs fd	Alleles	Sequenced clonesa	SNVs	nsSNVs	nsSNVs fd	Alleles
Cultured samples, no clinical data available
P344	29	46	12	3	21	19	45	31	5	14
P387	26	42	9	4	8	14	31	16	2	5
P413	20	15	4	1	1	20	9	6	1	1
P424	19	14	3	1	1	20	9	6	1	1
P427	28	44	9	3	9	15	35	17	5	8
P433	19	2	0	0	3	16	6	2	0	4
P458	24	3	0	0	4	20	6	2	0	3
Fecal sample, no clinical data available
0099	21	42	10	2	5	15	41	25	3	4
Fecal samples, clinically susceptible
Ag30	16	18	1	1	1	18	6	2	0	1
VA	20	23	3	1	1	19	16	12	4b	1
Ag15	6	31	4	1	2	16	29	16	3b	3
Fecal samples, clinically refractory
Ag10	16	42	8	2	4	14	28	13	2	6
Ag13	15	44	8	3	6	16	25	15	3	9
Ag20	8	25	2	1	2	6	19	10	2	3
Ag22	19	28	6	2	3	17	37	21	3	7

^aThe number of sequenced clones corresponds to the number of clones obtained after removal of chimeras (see Supplementary Table 2 for the total number of clones obtained).

^bSingle nucleotide deletions are included in the number.