Figure 3.

Isolated KLPJ induces colitis in pan-antibiotics–treated mice. (A) Schematic of the experiment for KLPJ-induced colitis. (B) Body weights of mice (n = 9). (C) Colon length in mice (n = 6). (D) ELISA of tumor necrosis factor α (TNFα), IL6, and IL18 in the colon tissues of mice (n = 6). (E) CFU of KLPJ or E coli in the colon contents of mice. For CFU in the bacteria-infused mice, 10⁹ bacteria were orally infused and then CFU were counted after 7 days. (F) Flow cytometry of CD11b⁺Ly6G⁺ cells in the colon tissues of mice (n = 5). Control indicates Ly6G isotypic control. (G) Flow cytometry of CD4⁺IFNγ⁺ cells in the colon tissues of mice (n = 5). Control indicates IFNγ isotypic control. (H) Flow cytometry of NKp46⁺IFNγ⁺ cells in the colon tissues of mice (n = 5). Control indicates isotypic control. (I) Flow cytometry of CD11b⁺MHCII⁺Ly6C⁺ inflammatory macrophages in the colon tissues of mice (n = 5). Control indicates isotypic control. Mice with (KLPJ or E coli) or without (PBS) gavage of KLPJ or E coli; PBS indicates control. (B) One-way analysis of variance test; (C–I), Student t test. ∗∗P < .01 and ∗∗∗P < .001. Data are representative of at least 2 experiments. FITC, fluorescein isothiocyanate; SSC,SideScatter.