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. 2022 Nov 25;15(3):613–632. doi: 10.1016/j.jcmgh.2022.11.005

Figure 4.

Figure 4

Isolated KLPJ promotes DSS-mediated colitis. (A) Schematic of the experiment for the effects of KLPJ on DSS-mediated colitis. (B) Survival rate of KLPJ or E coli–infused mice after DSS (n = 12). (C) Body weight of KLPJ or E coli–infused mice after DSS (n = 12). (D) DAI of mice after DSS (n = 12). (E) Colon length of mice after DSS (n = 6). (F) ELISA of tumor necrosis factor α (TNFα), IL6, and IL18 in the colon tissues of mice (n = 6) after DSS. (G) Flow cytometry of CD4+IFNγ+ cells in the colon tissues of mice after DSS (n = 6). Control indicates IFNγ isotypic control. (H) Flow cytometry of CD11b+MHCII+Ly6C+ inflammatory macrophages in the colon tissues of mice after DSS (n = 6). Control indicates isotypic control. (I) H&E staining of colon tissues of mice after DSS (3 slides/mouse, n = 6). Mice with (KLPJ or E coli) or without (PBS) the gavage of KLPJ or E coli. PBS indicates control. Dashed outline indiates that H/E staining on the right is from this region. Scale bar: 40 μm. (J) Schematic of the experiment for the effects of KLPJ on SPF WT or antibiotics-treated mice in DSS-mediated colitis. (K) Colon length of SPF after gavage using KLPJ or pan-antibiotics–treated mice after DSS (n = 6). (L) ELISA of TNFα, IL6, and IL18 in the colon tissues of SPF mice after gavage by KLPJ or pan-antibiotics–treated mice (n = 6) after DSS. (M) Colon length of pan-antibiotics–treated mice after gavage by E coli and/or KLPJ after DSS (n = 6). (N) ELISA of TNFα, IL6, and IL18 in the colon tissues of pan-antibiotics–treated mice after gavage by E coli and/or KLPJ after DSS (n = 6). (B) Wilcoxon test; (C and D) 1-way analysis of variance test; (E–H and K–N) Student t test; (I) Mann–Whitney U test. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001. Data are representative of at least 2 experiments. FITC, fluorescein isothiocyanate; Unt. C, untreated healthy mice.