Figure 5.

KLPJ-mediated colitis depends on IL18. (A) Schematic of the experiment for the effects of IL18 blocking reagent (IL18 Binding protein isoform d) on KLPJ-mediated colitis. (B) Colon lengths in mice (n = 6). (C) ELISA of tumor necrosis factor α (TNFα), IL6, and IL18 in the colon tissues in mice (n = 6). (D) Flow cytometry of CD11b⁺Gr1⁺, CD4⁺IFNγ⁺, and MHCII⁺Ly6C⁺ cells in mice (mixed sample per 2 mice, n = 6). (E) CFU of KLPJ in the colon contents of mice. For CFU in bacteria-infused mice, 10⁹ bacteria were orally infused and then CFU were counted after 7 days. (F) Schematic of the experiment for KLPJ-mediated colitis in WT and IL18 KO mice. (G) Colon lengths of WT and IL18 KO mice (n = 6). (H) ELISA of IL18 in the colon tissues in WT and IL18 KO mice (n = 6). (I) Flow cytometry of immune cells in the colon tissues of WT and IL18 KO mice (mixed sample per 2 mice, n = 6). (J) CFU of KLPJ in the colon contents of WT and IL18 KO mice. For CFU in bacteria-infused mice, 10⁹ bacteria were orally infused and then CFU were counted after 7 days. (B–F) Mice with (WT+18BPd+PBS or WT+18BPd+KLPJ) or without (WT+BPS or WT+KLPJ) IL18 blocking reagent (IL18BPd) and with (WT+18BPd+KLPJ or WT+KLPJ) or without (WT+18BPd+PBS or WT+BPS) KLPJ (KLPJ); (H–L) WT and IL18 KO mice with (WT+KLPJ or IL18KO+KLPJ) or without (WT+BPS or IL18KO+BPS) gavage of KLPJ. Student t test. ∗∗P < .01 and ∗∗∗P < .001. Data are representative of at least 2 experiments.