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. 2022 Nov 25;15(3):613–632. doi: 10.1016/j.jcmgh.2022.11.005

Figure 7.

Figure 7

Transplantation of WT or IL18 KO bone marrow cells does not affect KLPJ-mediated colitis. (A) Schematic of the effects of bone marrow cell (BMC) transplantation on KLPJ-mediated colitis. (B) Survival rates in BMC transplanted WT or IL18 KO mice after DSS (n = 12). (C) Body weight changes in BMC transplanted WT or IL18 KO mice after DSS (n = 12). (D) Disease activity index in BMC transplanted WT or IL18 KO mice after DSS (n = 12). (E) Colon length in BMC transplanted WT or IL18 KO mice after DSS (n = 6). (F) ELISA of tumor necrosis factor α (TNFα), IL6, and IL18 in the colon tissues in BMC transplanted WT or IL18 KO mice (n = 6) after DSS. (G) Flow cytometry (upper) and immunostaining (lower) of CD45.1 or CD45.2 cells in the spleens of IL18KO/WT (CD45.1) or WT/IL18KO (CD45.2) mice (n = 6) using APC-labeled CD45.1 or PE-labeled CD45.2. Scale bar: 40 μm. (H) Colon length in WT and IL22 KO mice (n = 6). (I) ELISA of IL18 in the colon tissues in WT and IL22 KO mice (n = 6). IL18KO/WT, BMCs from CD45.1 WT into irradiated CD45.2 IL18 KO mice; WT/IL18KO, BMCs from CD45.2 IL18 KO mice into irradiated CD45.1 WT mice; IL18KO/IL18KO, BMCs from IL18 KO mice into irradiated IL18 KO mice; WT/WT, BMCs from WT mice into irradiated WT mice. BMC transplanted mice then were infused with KLPJ, and treated using DSS. (B) Wilcoxon test; (C and D) 1-way analysis of variance test; and (E–I) Student t test. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001. Data are representative of at least 2 experiments. SSC, SideScatter; Unt. ctr., untreated control healthy mice.