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. 2022 Nov 25;15(3):613–632. doi: 10.1016/j.jcmgh.2022.11.005

Figure 9.

Figure 9

KLPJ-mediated IL18 in colon epithelial cells depends on caspase-1/11. (A) Immunoblotting of pro-mature and mature IL18 in the colon epithelial cells after exposure to alive KLPJ (KLPJ), killed KLPJ (killed KLPJ), and E coli. Control indicates PBS. (B) ELISA of IL18 in the supernatants of the colon after exposure to different concentrations of KLPJ. (C) Immunoblotting of pro- and mature caspase-1 and caspase-11 in the crypt supernatants, crypts, lamina propria, and colon after exposure to KLPJ. (D) ELISA of IL18 in the supernatants of the colon of WT, caspase-1/11 KO, NLRP3 KO, and NLRC4 KO mice after exposure to KLPJ. The colons were isolated from different mice, and then exposed to KLPJ. (E) Immunoblotting of pro- and mature IL18 in the crypt supernatants, crypts, lamina propria, and colon of WT caspase-1/11 KO mice after exposure to KLPJ. Actin was used as a loading control. Student t test. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001. sup, supernatatnts.