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. 2023 Jan 10;16:1097467. doi: 10.3389/fnana.2022.1097467

Figure 7.

Figure 7

Immunohistochemical labeling of the fox olfactory limbus (OL) using anti-Gαo. (A) Immunolabeling for the G-protein subunit Gαo revealed uniform expression of Gαo throughout the frontal lobe of the telencephalon (FL) and the olfactory bulb, but no immunostaining in the nerve or glomerular layers of the accessory olfactory bulb (AOB). Atypical glomeruli proximal to AOB (arrowheads) are more intensely immunolabeled with anti-Gαo than typical glomeruli in the main olfactory bulb (MOB). The macroglomerular complex (MGC, box B) shows a labeling intensity similar to the typical MOB glomeruli. (B) Higher magnification of box (B), showing that the MGC possesses two clearly differentiated areas: one containing numerous neuronal somata (black asterisk) and one devoid of somata (blue asterisk). Somata in the MGC are Gαo-immunopositive, unlike the mitral cell somata in the MOB, which are immunonegative (box C, C). EPL, external plexiform layer; GrL, granular layer. Scale bars: (A): 500 μm. (B,C): 125 μm.