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. 2023 Jan 10;16:1097467. doi: 10.3389/fnana.2022.1097467

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Copyright © 2023 Ortiz-Leal, Torres, Vargas-Barroso, Fidalgo, López-Beceiro, Larriva-Sahd and Sánchez-Quinteiro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemical labeling of the fox olfactory limbus (OL) with anti-microtubule-associated protein 2 (MAP-2). (A) Immunostaining with anti-MAP-2 results in the strong and uniform immunolabeling of glomeruli (Gl) and the external plexiform layer (EPL) of the main olfactory bulb (MOB). (B) A higher magnification view of the rostral part of the OL shows the staining of atypical Gl formations (open triangles), in addition to a patch of the nervous formation that remains unstained (*). (C) Anti-MAP-2 immunostained serial sections were counterstained with hematoxylin, confirming that the unlabeled area corresponds to an area of the macroglomerular complex that is rich in neuronal bodies (arrowheads) and receives the innervation from Ulex europaeus agglutinin-positive fibers. AOB, accessory olfactory bulb; AON, anterior olfactory nucleus; EPL, external plexiform layer; FL, front lobe of the telencephalon; Gl, glomeruli; MOB, main olfactory bulb; OL, olfactory limbus. Scale bars: (A): 1 mm. (B): 250 μm. (C): 100 μm.