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. 2022 Sep 13;16(2):195–217. doi: 10.1111/1751-7915.14135

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© 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Strategies for identification of novel plastic‐active enzymes. (A) Using Hidden Markov Model (HMM)‐based searches in combination with in vitro transcription and translation of targets can be one of the fastest strategies to obtain novel enzymes. (B) Using reporter strains that are able to detect terephthalic acid (TPA) uptake can be a highly sensitive way to detect polyethylene terephthalate (PET)‐degrading activity. Image shows a recombinant Comamonas sp. strain E6 carrying a thpA:sfGFP (tetrahydrophthalic anhydride/superfolding variant of green fluorescent protein (GFP)) promoter fusion in the presence or absence of 50 μm TPA. Images contain unpublished data from our laboratory.