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. 2023 Jan 10;13:1078526. doi: 10.3389/fimmu.2022.1078526

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Copyright © 2023 Li, Wang, Yu, Wang, Cao, Ma and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original authors(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of the bactericidal activity of duGSDME-NT. ETEC (F5+) was incubated with different amounts of purified His-rduGSDME-NT for 30 min and then spread on plates. The number of colonies was counted 24 hours later. ETEC (F5+) treated with purified rPLO served as a control. The colony number of the 125 mg/L His-rduGSDME-NT treated ETEC was significantly less than that of 125 mg/L rPLO treated ETEC. The experiment was repeated thrice. The data obtained in these experiments were statistically analyzed by one-way ANOVA test and shown as means ± SE (*, p=0.05).