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. 2023 Jan 10;13:1078526. doi: 10.3389/fimmu.2022.1078526

Figure 9.

Figure 9

DHAV-3 challenge induces ducaspase-3 activation and duGSDME cleavage in ducklings. (A) duGSDME was expressed in the brain of the healthy duckling, although at an extremely low level. Expression of duGSDME-NT was detected in the heart and thymus of the healthy duckling. Ducaspase-3 was detected in the heart, brain and thymus of the healthy duckling, but no active ducaspase-3 was detected. DHAV-3 challenge resulted in the upregulation of full-length duGSDME in the bursa and duGSDME-NT in the liver, kidney, stomach and thymus of the duckling. DHAV-3 challenge also led to the upregulation of ducaspase-3 in the heart, liver, lung, kidney, stomach, thymus and bursa, and the activation of ducaspase-3 in the heart, liver and stomach of the duckling; (B) A 55 kDa protein in the brain of the healthy duckling was recognized by the anti-duGSDME-NT antibody. After the incubation with rduGSDME-His, the anti-duGSDME-NT serum failed to recognize the 55 kDa protein. In contrast, the incubation with rDHAV-3 VP1 and rPLO did not affect the recognition of the anti-duGSDME-NT serum to the 55 kDa protein.