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Primers used for generating mutation in the eukaryotic expression plasmids.
| Plasmids | Primer sequence |
|---|---|
| pET-30a(+)-duGSDME D270A | 5’- GTTTCTGTATCAGCCAGCTGCTGTTGATAATGGGA-3’ |
| 5’- TCCCATTATCAACAGCAGCTGGCTGATACAGAAAC-3’ | |
| pET-30a(+)-duGSDME D273A | 5’-CAGCCAGATGCTGTTGCTAATGGGATGTACTCTGG-3’ |
| 5’-CCAGAGTACATCCCATTAGCAACAGCATCTGGCTG-3’ | |
| pET-30a(+)-ducaspase-3 C170A | 5’-CTTCTTCATTCAGGCTGCTAGAGGGACAGAACTA-3’ |
| 5’TAGTTCTGTCCCTCTAGCAGCCTGAATGAAGAAG-3’ |
