Table 4.

Characteristics of the plasmids.

Plasmid names	Characteristics
pET-30a(+)-duGSDME	encodes a full length pGSDME, fused with His tag at the carboxyl terminal
pET-30a(+)-ducaspase-3	encodes a full length ducaspase-3, fused with His tag at the carboxyl terminal
pET-30a(+)-ducaspase-7	encodes a full length ducaspase-7, fused with His tag at the carboxyl terminal
pET-30a(+)-duGSDME-NT	encodes the 1-273 aa fragment of duGSDME (duGSDME-NT), fused with His tag at the amino terminal
pcDNA3.1(+)-ducaspase-3	encodes a full length ducaspase-3
pcDNA3.1(+)-ducaspase-7	encodes a full length ducaspase-7
pECMV-3×FLAG-N-duGSDME	encodes a full length duGSDME, fused with 3×FLAG tag at the amino terminal
pcDNA3.1(+)-duGSDME-NT	encodes the 1-273 aa fragment of duGSDME (duGSDME-NT)
pcDNA3.1(+)-duGSDMD-CT	encodes the 274-499 aa fragment of duGSDME (duGSDME-CT) (pGSDME-NT)
pET-30a(+)-duGSDME D270A	encodes a full length duGSDME, the Asp270 of the duGSDME is replaced with Ala, fused with His tag at the carboxyl terminal
pET-30a(+)-duGSDME D273A	encodes a full length duGSDME, the Asp273 of the duGSDME is replaced with Ala, fused with His tag at the carboxyl terminal
pET-30a(+)-ducaspase-3 C170A	encodes a full length ducaspase-3, the Cys273 of the duGSDME is replaced with Ala, fused with His tag at the carboxyl terminal