Fig. 3.

FGFR4 silencing in liver promotes bile acids synthesis. A: Hepatic gene expression of Cyp7A1. Measure of serum bile acids (B) and serum cholesterol (C). Measure of primary (D) and secondary (E) bile acids species in the cardiac blood. Data are expressed as mean ± SEM (n = 4–8, ns = non-significant, ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗∗P < 0.0001, two-way ANOVA followed by a Tukey’s multiple comparisons test). Data were normalized by the control condition in chow diet mice for (A, D, and E). F: Heatmap of cholesterol and bile acids biosynthesis genes expression. G: Heatmaps of genes involved in bile acids transport. Each column represents a mouse (n = 4, ∗ P < 0.05; ∗∗ P<0.01; ∗∗∗ P<0.001; moderated t test). FGFR, fibroblast growth factor receptor.