Fig. 5.

FGFR4 KD increases hepatic IRS1 expression. Measure of Irs1 gene expression (A) and protein levels (B) in mice liver. Data are expressed as mean ± SEM (n = 4–8, two-way ANOVA followed by a Tukey’s multiple comparisons test). Data were normalized by the control condition in chow diet mice. C: Immunoblotting of IRS1 from liver biopsies. Measure of Fgfr4 gene expression (D) and protein levels (E) and Irs1 (G) gene expression in primary hepatocytes treated with 1000 ng/ml of an siRNA or a placebo in the presence or not of a mix of oleic acid and palmitic acid. F: Immunoblotting of FGFR4 from primary hepatocytes. H: Immunoblot of IR, AKT, and ERK phosphorylation in primary hepatocytes after insulin (10 nM) stimulation in the presence or not of palmitic acid. Data are expressed as mean ± SEM (n = 3–6, ns = non-significant, ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗P < 0.0001, two-way ANOVA followed by a Tukey’s multiple comparisons test). Data of each FGFR4 group were normalized by their own control for the figure D and G. FGFR, fibroblast growth factor receptor; IR, insulin receptor; KD, knockdown.