FIG. 1.

Indirect immunofluorescence microscopy of Arp3, VASP, and N-WASP in Cryptosporidium-infected cell lines. Parasites are identified by differential interference contrast (DIC) microscopy (A, D, H) and DAPI staining of parasite nuclei (B and E). The f-actin accumulation at these sites of infection is identified by fluorescein isothiocyanate-phalloidin staining (F and I). Indirect immunofluorescence microscopy of Cryptosporidium-infected cell lines reveals localization of Arp3 (C) and VASP (G) at all sites of infection. Indirect immunofluorescence confocal microscopy of the host-parasite interface in a cell line transfected with Myc-tagged N-WASP reveals a circular staining pattern at the periphery of the junctional complex (J). Scale bars = 5 μm.