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SDS-PAGE analysis of the two BsAbs compared to parental mAbs. Purified pertuzumab, anti-EGFR/HER2 BsAb, rituximab and anti-CD20/CD20 BsAb were subjected to electrophoresis in 4-12% SDS PAGE under (A) nonreducing condition and (B) reducing condition then stained with Coomassie Blue. Precision Plus Protein Kaleidoscope Prestained Protein Standards (Bio-Rad) was used as molecular weight marker.
