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Separation of antibodies by size exclusion chromatography. The elution profiles showed a major peak of (A) anti-EGFR/HER2 BsAb, (B) pertuzumab, (C) anti-CD20/CD20 BsAb, and (D) rituximab with a retention time that is consistent with their expected molecular weight of around 144-145 kDa, respectively. All SEC experiments were done in triplicate. Chromatograms shown here are representative of the results.
