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Far-UV CD spectrum of antibodies. (A) The spectrum of anti-EGFR/HER2 BsAb and its parental mAb (pertuzumab) show a negative band at around 218 nm. (B) The anti-CD20/CD20 BsAb and its parental mAb (rituximab) also show a similar spectrum of negative band as anti-EGFR/HER2 BsAb at approximately 218 nm. All proteins were buffer exchanged in milli-Q water prior analysis and done in triplicate.
