Table 1.

Primer sequences and annealing temperatures for polymerase chain reactions.

Gene	Primer sequence (5`-3`)	Product size (bp)	Annealing temp. (°C)
IL-18	Forward: TGAAAACGATGAAGACCTGGAA	101	60
	Reverse: CCTGGTTAATGAAAAGGACTTGG		60
IL-6	Forward: ATGAGAAGTGTGAAAACAGCAAGG	294	60
	Reverse: CATTTGTGGTGGGGTTAGGG		60
IFN-β	Forward: TCGCTCTCCTGATGTGTTTCTC	82	60
	Reverse: AAATTGCTGCTCCTTTGTTGGT		60
TNF-α	Forward: GGCGTGAAGCTGAAAGACAAC	127	60
	Reverse: GGCTGATGGTGTGAGTGAGG		60
IL-1β	Forward: AAGTGGTGTTCTGCATGAGCTTT	125	60
	Reverse: CAGGGTGGGCGTGTTATCTT		60
IFN-α	Forward: ACCTCAGCCAGGACAGCAGTATC	121	60
	Reverse: TCGCAGCCCAGAGAGCAGATG		60
GAPDH	Forward: TGTGTCCGTCGTGGATCTGA	150	60
	Reverse: TTGCTGTTGAAGTCGCAGGAG		60
27F	Forward: AGAGTTTGATCMTGGCTCAG	1900	60
1492R	Reverse: GGTTACCTTGTTACGACTT		60