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. 2022 May 24;14(1):28–36. doi: 10.1093/procel/pwac013

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©The Author(s) 2022. Published by Oxford University Press on behalf of Higher Education Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Flow chart of screening and confirmatory procedure and results. A total of 88 517 blood donations were donated between 1 September and 31 December 2019 in Wuhan. Of these, 43 850 achieved samples from 32 484 blood donors were qualified for further pan-Ig testing. 264 pan-Ig reactive samples from 213 blood donors were further tested IgG and IgM antibodies to SARS-CoV-2 and we found 51 reactive (from 38 blood donors): 8 were IgG reactive and 43 were IgM reactive. These 264 samples were finally confirmed negative by microneutralization assay. Neutralizing antibody titers of all samples were <1:8. R, reactive; NR, non-reactive; pan-Ig, pan-immunoglobulins to SARS-CoV-2; IgG, IgG antibody against receptor-binding domain (RBD) of the spike protein of SARS-CoV-2; IgM, IgM antibody against RBD antigen of SARS-CoV-2; MN, microneutralization assay.