Table 1.
MBD variants | Protein Prep Yield* (mg) | Cm (M) | m (kcal mol−1 M−1) | (kcal mol−1 ) | (kcal mol−1 ) | (kcal mol−1) |
---|---|---|---|---|---|---|
WT | 1.0 | 4.1 ± 0.2 | 0.54 ± 0.04 | 2.07 ± 0.15 | ||
T158M | 0.9 | 3.8 ± 0.3 | 0.43 ± 0.02 | 1.71 ± 0.09 | 0.36 | 0.14 |
R133C | 0.7 | 3.7 ± 0.2 | 0.41 ± 0.05 | 1.55 ± 0.18 | 0.52 | 0.21 |
R106W | 0.8 | 4.0 ± 0.2 | 0.47 ± 0.03 | 2.02 ± 0.12 | 0.05 | 0.03 |
P152R | 0.4 | 0.8 ± 0.3 | 1.01 ± 0.14 | 0.97 ± 0.14 | 1.10 | 1.82 |
A140V | 1.4 | 5.2 ± 0.4 | 0.53 ± 0.03 | 2.8 ± 0.16 | −0.73 | −0.61 |
S134C | 0.4 | 2.0 ± 0.3 | 0.56 ± 0.11 | 1.13 ± 0.23 | 0.94 | 1.15 |
R106Q | 0.7 | 3.9 ± 0.2 | 0.51 ± 0.02 | 1.91 ± 0.09 | 0.16 | 0.08 |
D156E | 0.4 | 2.8 ± 0.2 | 0.41 ± 0.04 | 1.03 ± 0.09 | 1.04 | 0.71 |
R133H | 0.8 | 3.7 ± 0.3 | 0.58 ± 0.07 | 1.83 ± 0.23 | 0.24 | 0.23 |
L100V | 0.5 | 1.9 ± 0.2 | 0.67 ± 0.05 | 1.08 ± 0.09 | 0.99 | 1.22 |
F155S | 0.3 | 3.2 ± 0.3 | 0.44 ± 0.03 | 1.70 ± 0.10 | 0.37 | 0.49 |
T158A | 1.2 | 2.7 ± 0.3 | 0.54 ± 0.05 | 1.90 ± 0.17 | 0.17 | 0.77 |
R111G | 1.6 | 4.7 ± 0.2 | 0.62 ± 0.06 | 2.59 ± 0.23 | −0.52 | −0.33 |
The protein prep yield was estimated by the final concentration and total volume from 500 ml cell culture.