Table 1.

Equilibrium denaturation of MBD for WT (Wild-type) and mutants

MBD variants	Protein Prep Yield* (mg)	Cm (M)	m (kcal mol−1 M−1)	${\mathit{\Delta }\mathit{G}}_{\mathit{a}\mathit{p}\mathit{p}}^{{\mathit{H}}_2\mathit{O}}$(kcal mol−1 )	${\mathit{\Delta }\mathit{\Delta }\mathit{G}}_{\mathit{a}\mathit{p}\mathit{p},1}^{{\mathit{H}}_2\mathit{O}}$(kcal mol−1 )	${\mathit{\Delta }\mathit{\Delta }\mathit{G}}_{\mathit{a}\mathit{p}\mathit{p},2}^{{\mathit{H}}_2\mathit{O}}$(kcal mol−1)
WT	1.0	4.1 ± 0.2	0.54 ± 0.04	2.07 ± 0.15
T158M	0.9	3.8 ± 0.3	0.43 ± 0.02	1.71 ± 0.09	0.36	0.14
R133C	0.7	3.7 ± 0.2	0.41 ± 0.05	1.55 ± 0.18	0.52	0.21
R106W	0.8	4.0 ± 0.2	0.47 ± 0.03	2.02 ± 0.12	0.05	0.03
P152R	0.4	0.8 ± 0.3	1.01 ± 0.14	0.97 ± 0.14	1.10	1.82
A140V	1.4	5.2 ± 0.4	0.53 ± 0.03	2.8 ± 0.16	−0.73	−0.61
S134C	0.4	2.0 ± 0.3	0.56 ± 0.11	1.13 ± 0.23	0.94	1.15
R106Q	0.7	3.9 ± 0.2	0.51 ± 0.02	1.91 ± 0.09	0.16	0.08
D156E	0.4	2.8 ± 0.2	0.41 ± 0.04	1.03 ± 0.09	1.04	0.71
R133H	0.8	3.7 ± 0.3	0.58 ± 0.07	1.83 ± 0.23	0.24	0.23
L100V	0.5	1.9 ± 0.2	0.67 ± 0.05	1.08 ± 0.09	0.99	1.22
F155S	0.3	3.2 ± 0.3	0.44 ± 0.03	1.70 ± 0.10	0.37	0.49
T158A	1.2	2.7 ± 0.3	0.54 ± 0.05	1.90 ± 0.17	0.17	0.77
R111G	1.6	4.7 ± 0.2	0.62 ± 0.06	2.59 ± 0.23	−0.52	−0.33

^*The protein prep yield was estimated by the final concentration and total volume from 500 ml cell culture.