Fig. 7.

Therapeutic effect of microRNA (miR)-9-5p in destabilized medial meniscus (DMM)-induced osteoarthritis (OA) mice. a) Radiographs and b) micro-CT imaging for morphological structure in knee of OA mouse at 12 weeks post-DMM surgery, followed by treatment with intra-articular (IA) injection of exosomes with high miR-9-5p expression and miRNA-NC, respectively (n = 4). c) and d) Haematoxylin and eosin (H&E) staining and Safranin-O/Fast Green staining of articular cartilage tissues of OA mouse at 12 weeks post-DMM surgery, followed by treatment with IA injection of exosomes with high miR-9-5p expression and miRNA-NC, respectively (n = 4; scale bar: 100 μm). e) and f) Results of Safranin-O/Fast Green staining of e) Osteoarthritis Research Society International (OARSI) score and f) depth of cartilage lesions (n = 4). g) Immunohistochemistry staining showed the intensity of immunostaining of COL2α1 and matrix metalloproteinase (MMP)-13 of OA mouse at 12 weeks post-DMM surgery, followed by treatment with IA injection of exosomes with high miR-9-5p expression and miRNA-NC, respectively (n = 4; scale bar: 100 μm). Independent-samples t-test was used to compare data between two groups, and one-way analysis of variance was used for comparison between multiple groups. NC, negative control.