Skip to main content
. Author manuscript; available in PMC: 2023 Dec 28.
Published in final edited form as: ACS Appl Mater Interfaces. 2022 Dec 16;14(51):56440–56453. doi: 10.1021/acsami.2c14424

Figure 3.

Figure 3.

Transfection of GFP encoded pDNA and mRNA in RAW 264.7 cells. (A) RAW 264.7 cells were transfected with polyMDET/pDNA or polyMDET-Cp/pDNA and polyMDET/mRNA or polyMDET-Cp/mRNA polyplexes prepared at a weight ratio of 110:1. GFP was used to assess for the transfection efficiency of the polyplexes. Scale bar 100 μm. (B) Representative gating strategy for the quantification of transfection by flow cytometry. Flow cytometry-based quantification of transfection efficiency of (C) polyMDET/pDNA and polyMDET-Cp/pDNA and (E) polyMDET/mRNA, polyMDET-Cp/mRNA polyplexes, respectively. Mean fluorescence intensity (MFI) of GFP expression after transfection of (D) GFP-encoded pDNA and (F) mRNA, respectively. PEI30 corresponds to polyplexes prepared at N/P ratio 30. The amount of GFP pDNA and GFP mRNA used was 2 μg and 1 μg, respectively per 2 × 105 RAW 264.7 cells. Data are representative of n=3 experiments. Statistical differences between groups were determined by performing a one-way ANOVA and Tukey’s post-hoc test (**p<0.01, ****p<0.0001). Errors bars represent standard deviation.